Workshop on biostatistics and bioinformatics
The Unit of Functional Pharmacology, Uppsala University, provided training for LIOS researchers in the basic usage of R programming languages and biostatistics, which serve as useful tools in the management of large-scale data analysis. Lectures and practical tasks were used to familiarize researchers with R and RStudio software. During the course researchers got the introduction to basic commands and practiced in script writing. Moreover, researchers developed a firm grasp of basic statistics as well as how to practically implement them in statistical packages. Taking into account large data is being stored in databases, gained bioinformatics skills gave LIOS reesearchers a solid understanding of the vast array of different types of databases that are available and on how to interpret different types of results.
Workshop on brain energy metabolism in emotion and cognition
The workshop was hosted by Eberhard Karls Universitaet Tuebingen in collaboration with Universitaetsklinik Jena. The lectures focused on the brain function, metabolism and inflammation were given by the researchers from Universitaetsklinik Jena, Eberhard Karls Universitaet Tuebingen and invited speakers. 63 participants from partner instutions and collaborators found out about brain metabolism and inflammation crosstalk, and how state-of-the-art neuroimaging techniques can help in the investigation of the neuronal changes induced by mental disorders as well as through drug challenge.
Virtual training on combined FluoRespirometry
In addition to respirometric parameters, the Oxygraph-2k allows the simultaneous detection of fluorometric signals. Training on fluorometry for early stage researchers was held in 2 parts in June and August, 2021. The scientific team of the Oroboros Instruments provided theoretical and practical support.
On June 14th the training was addressed to the measurements of the production of hydrogen peroxide using the AmplexRed assay system.
On August 24-25th participants learned how to measure alterations of mitochondrial membrane potential using different fluorescence probes, and the release of calcium from mitochondria determined by Calcium-green.
It should be noted, that shortly after the training researchers from Latvian Institute of Organic Synthesis and Uppsala University performed first experiments applying novel knowledge and skills for research.
FAT4BRAIN Online workshops
June was busy month for our FAT4BRAIN project - 2 workshops took place online
On June 8th WP1 Workshop on central regulatory mechanisms of energy metabolism hosted by Uppsala University team took place. During the workshop the lecture on latest findings and up-to-date technologies on the energy metabolism research in brain were given by the researchers from Uppsala University, Eberhard Karls Universitaet Tuebingen and invited speakers. 45 participants of workshop found out what are metabolic abnormalities in major depressive disorders, how LEAP2 regulates ghrelin system, and why Drosophila is useful modle to study energy metabolism in CNS.
On June 18th 2nd workshop in series of workshops on career development “How to become a successful researcher” took place. The workshop was dedicated to Innovation and how to develop Innovation thinking using NABC model, as well as Teamwork in Project Management and what are critical points in teamwork development and how to deal with it.
Virtual training on high-resolution respirometry
During training young investigators performed instrument set-up and experiment with permeabilized cells on O2ks under supervision provided online by Oroboros Instruments exprienced researcher.
Training also involved a basal education in respiratory concepts and approaches and specific training in the use of substrate-uncoupler-inhibitor-titration (SUIT) protocols for the evaluation of respiratory parameters in intact and permeabilised cells with focus on fatty acid metabolism assessment. Based on new protocols recently developed by the science team at Oroboros Intruments, the researcher learned how to apply HRFR in studies on cell culture, as well as preclinical and clinical samples.
Training on scientific writing
Prof. Helgi Schiöth leads the unit of Functional Pharmacology at Uppsala. His research involves investigating pharmacological, genetic and behavioural aspects as well as translational neurometabolic research and central regulation of food intake across species. Professor Schiöth has been continuously listed among the worlds “most influential researchers/scientific minds” based on citations from the last 10 years. His publication and citation summary comprise a total of 411 papers in peer-reviewed journals on PubMed with over 360 original articles and 50 reviews and more than 30,000 citations overall. He published in highly ranking journals such as PNAS, Alzheimers & Dementia, Nature Reviews Neuroscience and Nature Reviews Drug Discovery.
With his great experience in this area, we are happy to announce that Prof. Helgi Schiöth will be holding the FAT4BRAIN workshop with the topic scientific writing on October 22, 2020. The workshop will be divided into two sections, a morning session with focus on evaluation techniques for junior scientists and an afternoon session with a lecture about scientific writing and Q/A for both junior and senior scientists. Evaluation of scientific texts is a key challenge within scientific writing and effective self evaluation is very important. Furthermore, scientific writing is not only about writing scientific articles, it is in general important for how research is communicated effectively. The purpose of this FAT4BRAIN workshop is to increase awareness and recognition of what constitutes a good scientific writing and to point out common pitfalls and mistakes during the process.
FAT4BRAIN School on mitochondrial physiology
The aim of the school is to initiate and extend a network in mitochondrial physiology and train early-stage researchers in both the basics and cutting edge science of mitochondria. To emphasise the interaction between early-stage researchers and experienced lecturers there will be extensive question and answer sessions to discuss understanding and research problems in the role of mitochondria in CNS physiology and pathology.
Preliminary programme could be found here
The 1st online workshop for early-stage researchers
The workshop will focus on the three topics Reseach ethics, Intellectual property and Scientific communication, presented by experts on the respective field from Uppsala University. The Schedule of the workshop could be found here.
FAT4BRAIN kick-off meeting
All partners arrived to Riga with the aim to start work on the project and , discuss implementation. During the meeting, FAT4BRAIN Executive Board, External Advisory Board, Focus area leads were appointed. Work package leaders updated schedule of upcoming events. In addition, joint publication possibilities, including review related to fatty acid metabolite involvement in CNS physiology and pathology, were discussed.