Fatty acids and their intermediates and derivatives have been recognized as crucial mediators of brain health. Consequently, disturbances in brain fatty acid metabolism are highly implicated in different aspects of neuronal dysfunction.
The thematic area of FAT4BRAIN is focused on the pivotal and common role of mitochondria-related fatty acid metabolism for the development of neurological disorders, a topic of highest biomedical and societal concern. The concerted research activities aim at deciphering novel mechanisms and developing new treatment strategies against these devastating diseases.
Workshop on brain energy metabolism in emotion and cognitionWorkshop hosted with lectures and scientific discussion on brain metabolism took place on September 7th, 2021
The workshop was hosted by Eberhard Karls Universitaet Tuebingen in collaboration with Universitaetsklinik Jena. The lectures focused on the brain function, metabolism and inflammation were given by the researchers from Universitaetsklinik Jena, Eberhard Karls Universitaet Tuebingen and invited speakers. 63 participants from partner instutions and collaborators found out about brain metabolism and inflammation crosstalk, and how state-of-the-art neuroimaging techniques can help in the investigation of the neuronal changes induced by mental disorders as well as through drug challenge.
Virtual training on combined FluoRespirometryFAT4BRAIN held virtual training on high-resolution respirometry combined with fluorometry assays
In addition to respirometric parameters, the Oxygraph-2k allows the simultaneous detection of fluorometric signals. Training on fluorometry for early stage researchers was held in 2 parts in June and August, 2021. The scientific team of the Oroboros Instruments provided theoretical and practical support.
On June 14th the training was addressed to the measurements of the production of hydrogen peroxide using the AmplexRed assay system.
On August 24-25th participants learned how to measure alterations of mitochondrial membrane potential using different fluorescence probes, and the release of calcium from mitochondria determined by Calcium-green.
It should be noted, that shortly after the training researchers from Latvian Institute of Organic Synthesis and Uppsala University performed first experiments applying novel knowledge and skills for research.